中国组织工程研究与临床康复2011，Vol.15Issue(32)：5951-5956,6.DOI:10.3969/j.issn.1673-8225.2011.32.014
诱导人眼轮匝肌来源肌源干细胞向许旺细胞样细胞的分化
Differentiation potential of human Orbicularis oculi muscle-derived stem cells towards Schwann cells phenotype
摘要
Abstract
BACKGROUND: Muscle derived stem cells (MDSCs) can be isolated from human orbicularis oculi muscle and be differentiated to a Schwann cell phenotype which could eventually provide functional benefits for peripheral nerve repair. OBJECTIVE: To induce the differentiation of MDSCs into Schwann cell phenotype. METHODS: ①Under the support of microscope, we collected the discarded human Orbicularis oculi muscle resected in the upper eyelid blepharoplasty and isolate human-MDSCs within it with aid of tri-enzyme digestion and pre-plating technique, and then identify the cells by immunohistochemistry method. ②We isolated Schwann cells and identify the cells by immunohistochemistry method. Through half-harvest method, we would like to prepare conditioned medium from Schwann cell culture. ③We co-culture human-MDSCs with Schwann cell conditioned medium and the transdifferentiated cell morphology was investigated daily under microscope. The common used marker, S-100, GFAP and p75 were stained to identify Schwann cell phenotype with use of immunohistochemistry method. RESULTS AND CONCLUSION: ①We collected human Orbicularis oculi muscle sample from three young female volunteer with their consensus. Human-MDSCs were isolated from Orbicularis oculi muscle and have their desmin positively stained and Sca-1 was positively expressed. ②Schwann cells were isolated and identified with S-100 positively stained at the rate of (97.4±0.7)%. ③The isolated human-MDSCs were successfully transdifferentiated into Schwann cell-like cells with positive expression of S100, GFAP and p75, which would serve as a unanimous evidence of Schwann cell phenotype. Human-MDSCs could be transdifferentiated into Schwann cell-like cells when co-cultured within Schwann cell conditioned medium, which would serve as an alternative candidate for commonly studied Schwann cells in tissue engineering nerve graft.关键词
人肌源干细胞/眼轮匝肌/重睑成形术/分化/许旺细胞/周围神经损伤/再生医学分类
医药卫生引用本文复制引用
丁维进,张文俊,孙美庆,苏志达,李翠,刘安堂,江华..诱导人眼轮匝肌来源肌源干细胞向许旺细胞样细胞的分化[J].中国组织工程研究与临床康复,2011,15(32):5951-5956,6.基金项目
上海市基础研究重点课题(08JC1407100). （08JC1407100）
