中国组织工程研究与临床康复2011，Vol.15Issue(36)：6789-6792,4.DOI:10.3969/j.issn.1673-8225.2011.36.032
大鼠胚胎中脑多巴胺神经元的分离培养与鉴定
Isolation, culture and identification of rat embryonic mesencephalic dopaminergic neurons
摘要
Abstract
BACKGROUND: Nerve cell transplantation therapy is one of the most promising methods to cure Parkinson's disease. OBJECTIVE: To investigate the culture, isolation and identification of rat embryo mesencephalic dopaminergic neurons. METHODS: The mesencephalon was dissected from rat embryo at embryonic day 14 (E14). The brain tissue was triturated into a fine single-cell suspension. The cells were cultured with the medium containing Neurobasal, B27 supplement, ?-mercaptoethanol and fetal bovine serum. The cells were identified with RT-PCR as well as immunocytochemistry, respectively. RESULTS AND CONCLUSION: The cells survived well in the medium. RT-PCR and immunocytochemistry results showed mostly all neurons expressed molecular markers for dopaminergic neurons. The dopaminergic neurons derived from rat embryonic mesencephalon were successfully isolated and cultured, which will provide the foundation for the further research about cell therapy of Parkinson's disease.关键词
多巴胺/神经元/中脑/大鼠/细胞培养分类
基础医学引用本文复制引用
魏小兵,邓兴力,王应莉,杨智勇,李智高,刘如恩,李玉..大鼠胚胎中脑多巴胺神经元的分离培养与鉴定[J].中国组织工程研究与临床康复,2011,15(36):6789-6792,4.基金项目
云南省教育厅科学研究基金(09Y0153),课题名称:大鼠胚胎中脑多巴胺神经元的分离培养与鉴定. （09Y0153）
