中国组织工程研究与临床康复2011，Vol.15Issue(36)：6785-6788,4.DOI:10.3969/j.issn.1673-8225.2011.36.031
体外诱导羊膜来源间充质干细胞分化为血管内皮细胞
In vitro differentiation of human amniotic mesenchymal stem cells into vascular endothelial cells
施晶晶 1朱栋晓 2金伟东 1王晓彦 1庄瑞娟 1李拜红 1苗宗宁3
作者信息
- 1. 无锡市第三人民医院,心内科,江苏省无锡市,214041
- 2. 无锡市第三人民医院,心超室,江苏省无锡市,214041
- 3. 无锡市第三人民医院,研究所,江苏省无锡市,214041
- 折叠
摘要
Abstract
BACKGROUND: Whether human amniotic mesenchymal stem cells can differentiate into corresponding target cells after transplantation into different types of tissues? OBJECTIVE: To investigate the feasibility of differentiation from human amniotic mesenchymal stem cells into vascular endothelial cells in vitro induced by vascular endothelial cell growth factor. METHODS: Human amniotic mesenchymal stem cells were isolated, and then characterized by flow cytometry using a panel of monoclonal antibodies. Differentiation into endothelial-like cells was induced by cultivation of confluent cells in the presence of 2% fetal calf serum and 50 μg/L vascular endothelial growth factor. We characterized these cells by immunofluorescence staining for endothelial-specific markers KDR and the von Will brand factor. RESULTS AND CONCLUSION: Isolated human amniotic mesenchymal stem cells were positive for the markers CD105, CD29 and CD44 and negative for typical hematopoietic and endothelial markers CD34, CD45, CD106 and HLA-DR. The morphology of induced cells was obviously changed. Immunofluorescence staining analysis of the confluent cells in situ showed positive for endothelial-specific markers like KDR and the von Will brand factor. The human amniotic mesenchymal stem cells have the capability to differentiate into vascular endothelial cells in vitro.关键词
羊膜/间充质干细胞/移植/血管内皮细胞/分化分类
医药卫生引用本文复制引用
施晶晶,朱栋晓,金伟东,王晓彦,庄瑞娟,李拜红,苗宗宁..体外诱导羊膜来源间充质干细胞分化为血管内皮细胞[J].中国组织工程研究与临床康复,2011,15(36):6785-6788,4.
