中国组织工程研究与临床康复2011，Vol.15Issue(36)：6802-6806,5.DOI:10.3969/j.issn.1673-8225.2011.36.035
脑脊液体外培养骨髓间充质干细胞
Influence of cerebrospinal fluid on in vitro culture of bone marrow mesenchymal stem cells
沈忆新 1王鹏 2石恩东2
作者信息
- 1. 苏州大学附属第二医院骨科,江苏省苏州市,215004
- 2. 山东省交通医院,山东省济南市,250031
- 折叠
摘要
Abstract
BACKGROUND: Cell transplantation is a promising strategy for treatment of spinal cord injury. But the current experimental evidence about cerebrospinal fluid (CSF) effect on the cells is lack. OBJECTIVE: To study the CSF effects on the in vitro culture of bone marrow mesenchymal stem cells (BMSCs). METHODS: BMSCs were isolated and purified by density gradient centrifugation and adherent culture, and BMSCs were passaged in vitro. The third and fourth passage cells were cultured in CSD to observe cells growth conditions, and analyze some of its phenotypic characteristics. The fifth passage cells were cultured in L-DMEM containing fetal calf serum and CSF. RESULTS AND CONCLUSION: We had successfully and effectively isolated and purified BMSCs by density gradient centrifugation with adherent culture. The results of the identification were positive of CD90, CD106, CD71, CD29, while CD45 was negative. Cells cultured in CSF still had still morphology of BMSCs. But few cells were neural-like cells. The results of the identification were positive of CD90, CD106, CD71, CD29, while CD45 was negative. Neuron-specific enolase was weakly positive. Cells had a similar S-shaped growth curve, and the growth curves were similar. BMSCs can still proliferate in CSF, thus it indicate that these cells possess high adaptability to their growth environment.关键词
骨髓间充质干细胞/脑脊液/分离培养/表型/细胞鉴定分类
医药卫生引用本文复制引用
沈忆新,王鹏,石恩东..脑脊液体外培养骨髓间充质干细胞[J].中国组织工程研究与临床康复,2011,15(36):6802-6806,5.
