中国组织工程研究与临床康复2011，Vol.15Issue(32)：6027-6031,5.DOI:10.3969/j.issn.1673-8225.2011.32.031
体外高浓度葡萄糖培养及黄芪干预人外周血内皮祖细胞的数量和增殖与分化
Effects of high glucose and Astragalus Membranaceus on the number, proliferation, and differentiation of endothelial progenitor cells from human peripheral blood in vitro
摘要
Abstract
BACKGROUND: The quantity and impaired function of endothelial progenitor cells (EPCs) is the important link of diabetic vascular complications. Astragalus Membranaceus (AMI) can protect endothelium of dysfunction which caused by various reasons and can effectively protect the function of vascular endothelium. OBJECTIVE: To investigate the effects of high glucose and AMI on the number, proliferation, and differentiation of EPCs from human peripheral blood in vitro. METHODS: EPCs were incubated with various concentrations of glucose for 24 hours and with 30 mmol/L glucose for different periods. Simultaneously, EPCs were pre-incubated with 20 g/L AMI for 24 hours and then all the cells were incubated with 30 mmol/L glucose for 24 hours. RESULTS AND CONCLUSION: High glucose decreased the number of EPCs and abilities of EPCs proliferation and differentiation in a dose- and time-dependent manner (P ＜ 0.05 or 0.01). Pre-incubated with AMI could increase EPCs quantity and improve EPCs proliferative and differentiative capacity (P ＜ 0.05 or 0.01). AMI can increase the number and improve the capacity of proliferation and differentiation of EPCs cultured with high glucose.关键词
内皮祖细胞/黄芪/葡萄糖/增殖/分化分类
医药卫生引用本文复制引用
徐寒松,雷闽湘,孔德明,向慧,谢晓云..体外高浓度葡萄糖培养及黄芪干预人外周血内皮祖细胞的数量和增殖与分化[J].中国组织工程研究与临床康复,2011,15(32):6027-6031,5.基金项目
国家自然科学基金项目(30960491) （30960491）
贵州省优秀科技教育人才省长基金项目. （）
