中国组织工程研究与临床康复2011，Vol.15Issue(32)：6041-6044,4.DOI:10.3969/j.issn.1673-8225.2011.32.034
融合蛋白AML1-ETO对C/EBPα基因表达的影
Effects of AMLl-ETO fusion gene on the expression of C/EBPα
庄文越 1陈子兴 2赵昀 3岑建农 1沈宏杰 2祁小飞 1李炳宗2
作者信息
- 1. 苏州大学医学部,江苏省苏州市,215000
- 2. 苏州大学附属第一医院,江苏省血液学研究所,江苏省苏州市,215000
- 3. 北华大学医学检验学院,吉林省吉林市,132013
- 折叠
摘要
Abstract
BACKGROUND: Recent studies have shown that function of C/EBPa gene can be inhibited by AML1-ETO resulting in differentiation block. OBJECTIVE: To investigate the effects of AML1-ETO fusion gene on transcription factor C/EBPa which is crucial for the differentiation of granulocytes, and to explore its role in leukemogenesis. METHODS: The expression of cell differentiation antigen including CD11b and CD14 was detected by flow cytometry. C/EBPa gene expression was assessed by quantitative PCR, and C/EBPa protein expression was tested by Western blot. The chromatin immunoprecipitation (ChIP)-based PCR was used to investigate the direct interaction between the AML1-ETO and C/EBPa promoter in AML1-ETO positive leukemia cell line. RESULTS AND CONCLUSION: The expression of cell differentiation antigen CD11b and CD14 significantly decreased in transfected cells. C/EBPa expression in both mRNA and protein levels decreased in U937 cells transfected with pcDNA3.1-AML1-ETO. The enriched regions in transfected cells were located within C/EBPa promoter. Results indicated that C/EBPa is the direct target gene of AML1-ETO. AML1-ETO can down-regulate the expression of C/EBPa.关键词
融合蛋白/AML1-ETO/C/EBPα/基因表达/CD11b/CD14分类
基础医学引用本文复制引用
庄文越,陈子兴,赵昀,岑建农,沈宏杰,祁小飞,李炳宗..融合蛋白AML1-ETO对C/EBPα基因表达的影[J].中国组织工程研究与临床康复,2011,15(32):6041-6044,4.
