中国病理生理杂志2011，Vol.27Issue(10)：1938-1943,6.DOI:10.3969/j.issn.1000-4718.2011.10.016
川芎嗪与氨基胍联合治疗对n0-STZ大鼠肾功能的影响
Tetramethylpyrazine combined with aminoguanidine improves renal functions in n0-STZ Wistar rats
摘要
Abstract
AIM: To investigate the effects of tetramethylpyrazine combined with aminoguanidine on the renal functions of neonatal - 0 streptozotocin - induced ( nO - STZ ) rats. METHODS: Neonatal Wistar rats were intraperitoneal-ly injected with a single dose of streptozotocin ( STZ ) to establish the nO - STZ rat model. The nO - STZ rats were divided into 4 groups: normal control group, insulin resistance group, metformin treatment group and tetramethylpyrazine + aminoguanidine treatment group. Fasting plasm glucose, fasting insulin, insulin resistance index, blood urea nitrogen, serum creatinine, urine albumin and glomerular filtration rate were measured at the 32nd week. The mRNA content of inducible nitric oxide synthase ( iNOS ) in peripheral blood leukocytes was detected by the technique of in situ hybridization. Nitric oxide ( NO ) concentration, iNOS activity, the protein expression of iNOS and 3 - nitrotyrosine( 3 - NT ) were also assessed in the renal tissues. RESULTS: At the 8th week after the administration of STZ, 82. 5% of Wistar rats showed that the fasting plasm glucose level was ≥7. 0 mmol/L and the renal functions were seriously damaged. Although both metformin and the combined treatment reduced fasting plasm glucose, fasting insulin and insulin resistance index, the combined treatment was superior in improving the insulin resistance. The damaged renal functions were improved by the combined treatment as reducing blood urea nitrogen and creatinine, increasing glomerular filtration rate were observed. Furthermore, the combined treatment reduced NO concentration, decreased iNOS activity and diminished mRNA content of iNOS, resulting in depressing the generation of 3 - NT and iNOS, which surpassed the treatment of metformin. CONCLUSION: Tetramethylpyrazine combined with aminoguanidine improves the renal functions of nO - STZ rats by depressing nitrative stress and enhancing the effect of metformin.关键词
胰岛素抵抗/川芎嗪与氨基胍/一氧化氮/诱导型一氧化氮合酶/3-硝基酪氨酸Key words
Insulin resistance/Tetramethylpyrazine and aminoguanidine/Nitric oxide/Inducible nitric oxide synthase/3 - Nitrotyrosine分类
医药卫生引用本文复制引用
窦橙云,王蔚琛,曹创杰,魏树珍,李瑞峰..川芎嗪与氨基胍联合治疗对n0-STZ大鼠肾功能的影响[J].中国病理生理杂志,2011,27(10):1938-1943,6.基金项目
山东省自然科学基金资助项目(No.Y2006C27) （No.Y2006C27）
山东省卫生厅资助项目(No.99CA1CAA11) （No.99CA1CAA11）
