中国病理生理杂志2011，Vol.27Issue(10)：1967-1971,5.DOI:10.3969/j.issn.1000-4718.2011.10.021
Fractalkine对类风湿关节炎患者成纤维样滑膜细胞中NF-κB活化及内源性fractalkine mRNA表达的影响
Effects of fractalkine on nuclear factor-κB activation and endogenous fractalkine mRNA expression in fibroblast-like synoviocytes from patients with rheumatoid arthritis
摘要
Abstract
AIM: To investigate the effects of fractalkine( FKN ) on nuclear factor kappa B ( NF - kB ) activation and endogenous FKN mRNA expression in fibroblast - like synoviocytes ( FLS ) from the patients with rheumatoid arthritis ( RA ). METHODS: RA - FLS were gained through tissue culture. Fractalkine at 100 μg/L was used to stimulate RA - FLS for 0 h, 1 h and 2 h. The expression of NF - kB p65 protein in cytoplasm and nucleus was detected by Western blotting, representing the activation of NF - kB in RA - FLS. RA - FLS was stimulated with fractalkine at concentration of 100 μg/L for 0 h, 12 h or 18 h, and the mRNA expression of FKN in RA - FLS was detected by RT - PCR. RESULTS: After stimulated with recombinant human FKN for 1 h, the expression of NF - kB p65 protein in the cytoplasm of RA - FLS was obviously lower than that in RA - FLS without FKN treatment in control group ( P <0. 05 ). After stimulated with FKN for 2 h, the expression of NF - KBp65 protein in nucleus was obviously higher than that in RA - FLS of control group ( P < 0. 05 ). Recombinant human FKN at concentration of 100 μg/L induced endogenous FKN mRNA expression in RA - FLS in a time - dependent manner. The mRNA expression of FKN in RA - FLS obviously increased after stimulated with FKN for 18 h ( P <0. 05 ). CONCLUSION: FKN up - regulates the expression of endogenous FKN mRNA, suggesting a positive feedback. FKN can activate the NF - kB and may play an important role in the beginning of joint inflammation, angio-genesis and bone destruction.关键词
Fractalkine/关节炎,类风湿/成纤维样滑膜细胞/NF-κBKey words
Fractalkine/Arthritis,rheumatoid/Fibroblast - like synoviocytes/NF - kappa B分类
医药卫生引用本文复制引用
郭兴华,潘云峰,宋泽蓉,王昆,古洁若..Fractalkine对类风湿关节炎患者成纤维样滑膜细胞中NF-κB活化及内源性fractalkine mRNA表达的影响[J].中国病理生理杂志,2011,27(10):1967-1971,5.基金项目
广东省自然科学基金资助项目(No.9151008901000058) （No.9151008901000058）
