中国病理生理杂志2011，Vol.27Issue(10)：1989-1994,6.DOI:10.3969/j.issn.1000-4718.2011.10.025
mRSD-9基因参与细胞内吞功能的初步研究
Preliminary study of endocytosis-related gene mRSD-9
摘要
Abstract
AIM: To study the function of the gene mRSD -9. METHODS: The techniques of immunopre-cipitation and immunofluorescence were applied to verify the interaction between mRSD - 9 and endophlin 3. The ATP/ GTP combined experiment and the clathrin releasing experiment were conducted to investigate the effect of mRSD - 9 on en-docytosis. RESULTS: Expressed Myc - mRSD -9 was precipitated by Flag - endophilin 3. Conversely, the expressed Flag - endophilin 3 was also precipitated by Myc - mRSD - 9. Under laser scanning confocal microscope, mRSD - 9 - GFP fusion protein and endophilin 3 - RFP fusion protein were observed to co - localize in CHO cells. The combination of mRSD -9 protein with ATP/GTP was found and was specific because no combination was detected using mutant AmRSD -9. In empty vector transfected group, the quantity of transferrin in red fluorescent expressed cells was roughly the same as the un-transfected cells. In pDsRedl - N1 - mRSD - 9 transfected group, the quantity of transferrin in mRSD - 9 protein expressed cells was obviously reduced. In pDsRedl - Nl - AmRSD - 9 transfected group, the quantity of transferrin in AmRSD - 9 protein expressed cells was significantly increased. CONCLUSION: The protein coded by mRSD - 9 gene can suppress endocytosis of transferrin.关键词
基因,mRSD-9/Endophilin/3/胞吞作用Key words
Genes,mRSD - 9/Endophilin 3/Endocytosis分类
生物科学引用本文复制引用
李树春,王槐,王桂萍,满序聪..mRSD-9基因参与细胞内吞功能的初步研究[J].中国病理生理杂志,2011,27(10):1989-1994,6.基金项目
长江学者和创新团队发展计划资助项目(No.IRT0871) （No.IRT0871）
