中国病理生理杂志2011，Vol.27Issue(10)：2035-2039,5.DOI:10.3969/j.issn.1000-4718.2011.10.036
MT1-MMP基因敲除细胞的诱导多能干细胞系的构建
Induction of pluripotent stem cells from MT1-MMP-deficient cells
摘要
Abstract
AIM: To determine the difference of biological potency between induced pluripotent stem cells ( iP-SCs ) and embryonic stem cells ( ESCs ) by investigating the induction of reprogramming of wild type ( WT ) and membrane - type 1 matrix metalloproteinase ( MT1 - MMP ) - deficient mouse fibroblasts into iPSCs in vitro. METHODS: WT and MT1 - MMP - deficient mouse fibroblasts were reprogrammed to iPSCs by retroviral introduction of the 4 transcription factors Oct3/4, Sox2, c - Myc and Klf4. The expression of ESC - specific markers alkaline phosphatase ( AP ), stage specific embryonic antigen -1 ( SSEA - 1 ) and Oct3/4 was detected in iPSCs by immunocytochemistry. The method of " hanging drop" was applied to differentiate iPSCs into endothelial cells ( Ecs ) and cardiomyocytes for determining the differentiation potency of the cells. RESULTS: The formation of iPSCs colonies became visible approximately 2 weeks after the retroviral infection. The expression of ESC - specific markers AP, SSEA - 1 and Oct3/4 in iPSCs was obviously observed. The Ecs, which differentiated from iPSCs, expressed early endothelial marker Flk - 1/KDR. The expression of cardiomyocyte - typical marker cardiac troponin I in the cardiomyocytes differentiated from iPSCs was also detected by immunocytochemical staining when the beating cells were growing. CONCLUSION: WT and MT1 - MMP - deficient mouse fibroblasts can be re-programmed to iPSCs. The morphology, proliferation and multilineage differentiation potency of iPSCs are indistinguishable from those of ESCs, indicating that iPSCs will become optimal source of seed cells for regenerative medicine and specific cell therapy.关键词
胚胎干细胞/诱导多能干细胞/基因,MT1-MMPKey words
Embryonic stem cells/Induced pluripotent stem cells/Genes,MT1 - MMP分类
医药卫生引用本文复制引用
王彦霞,马玲,马海英,魏文娟,卢晓梅,金玉楠,于艳秋..MT1-MMP基因敲除细胞的诱导多能干细胞系的构建[J].中国病理生理杂志,2011,27(10):2035-2039,5.基金项目
国家自然科学基金资助项目(No.81101109) （No.81101109）
