中国生物防治学报2011，Vol.27Issue(2)：214-220,7.
海洋真菌的筛选及其对离体荔枝果霜霉病和炭疽病的防效
Screening of Marine Antagonistic Fungi and Their Control Efficiency on Downy Mildew and Anthracnose Diseases of Harvested Litchi Fruit
摘要
Abstract
Sixty-four strains of marine-derived fungi were isolated from the sediment and seawater collected from mangrove forest in Beibu Bay in China. Eight strains were demonstrated to be inhibitive against mycelial growth of both Perono-phythora litchii and Colletotrichum gloeosporioides. Fermentation filtrate of the strains MF-3、MF-13、MF-15 and MF-16 had high inhibition activity against mycelial growth of P. Litchii by over 94% inhibitory rate, and the strains MF-3 and MF-16 could restrain mycelial growth of C. Gloeosporioides by over 70% . Fruit preservation experiments demonstrated that browning index of the Iichi fruit treated by the fermentation filtrate of the five strains MF-3, MF-6, MF-8, MF-13 and MF-95 was lower than that of CK, and the strain MF-9 was the best on prevention of fruit browning. When Iichi fruits were immersed in the fermentation filtrate of MF-6、MF-8 and MF-9, separately, for 1 d and then inoculated with P. Litchii, disease incidence was 68.3 %, 71.67% and 66.18%, respectively, after 3d, significantly lower than that of control (87.2% ) or dimethomorph treatment (84.2%) ( P < 0.05) . The onset of fruit putrefaction could be post-poned 1 d in comparison with CK. After 3 d of inoculation with C. Gloeosporioides, disease incidence could be reduced to 64.44% by MF-6 treatment, without significant difference from that of zineb (62.54%) or badistan (62.76% ) treatment. According to morphological and molecular characteristics, strain MF-3、MF-6、MF-8、MF-9 and MF-13 were identified as Eupenicillium brefeldianum, Penicilllium janthinellum, Penicillium adametzii, Penicillium oxalicum and As-pergillus clavalonanicus, respectively.关键词
海洋真菌/荔枝/霜疫霉菌/炭疽病菌/防治效果Key words
marine fungi/ Litchi chinensis Sonn./ Peronophythora litchii/ Colletotrichum gloeosporioides/ control effi-ciency分类
生物科学引用本文复制引用
许兰兰,黄曦,李昆志,陈丽梅,黄庶识..海洋真菌的筛选及其对离体荔枝果霜霉病和炭疽病的防效[J].中国生物防治学报,2011,27(2):214-220,7.基金项目
国家自然科学基金(30860010) （30860010）
广西科学基金项目(桂科同0832006) （桂科同0832006）
广西科研院所基本科研业务费资助项目(08YJ16WL01) （08YJ16WL01）
广西留学人员科技活动项目(佳人函[2008]987号) （佳人函[2008]987号）
