中国生物防治学报2011，Vol.27Issue(2)：176-181,6.
对蛴螬有活性的苏云金芽胞杆菌菌株的筛选与基因鉴定
Screening and Identification of cry Gene from Bacillus thuringiensis Strains Toxic to the Larvae of Cockchafers
摘要
Abstract
To identify gene resources toxic to cockchafers, 500 strains of Bacillus thuringiensis isolated in our laboratory were investigated. Forty-three strains had insecticidal activity against cockchafers, and C9 was entomocidal to all the three species of cockchafers tested. Results of optical and scanning electronic microscope observation indicated that all of these Bt strains contained crystals in spherical shape. SDS-PAGE analysis indicated that all these strains could express protein with 130 kD molecular mass. Three methods were used to study the cry gene-types of these 43 strains. In 21 strains, there was only cry8Ca type gene, In 13 strains, cry8Ca and cry8Ea type genes co-existed. In 8 strains, PCR products of high-conserved region (about 110 bp) could be obtained only with primers HRM8F/HRM8R, indicating that it is quite different between the 5' flanking sequences of these genes with known cry8 genes. Results of sequencing and BLAST analysis indicated that the sequences similarity between the products and cry8 gene is more than 98% . However, the exact gene types need to be determined by further studies. Strain QCM may contain a novel cry8 gene for its unique PCR-RFLP patterns.关键词
蛴螬/苏云金芽胞杆菌/PCR-RFLP/cry8基因Key words
cockchafers/ Bacillus thuringiensis/ PCR-RFLP/ cry8 gene分类
农业科技引用本文复制引用
茅洁瑜,束长龙,李克斌,刘春琴,林毅,宋福平,张杰..对蛴螬有活性的苏云金芽胞杆菌菌株的筛选与基因鉴定[J].中国生物防治学报,2011,27(2):176-181,6.基金项目
转基因生物新品种培育科技重大专项(2009ZX08009-030B) （2009ZX08009-030B）
公益性行业(农业)科研专项(201003025) （农业）
国家"863"计划项目(2011AA10A203) （2011AA10A203）
