中华耳科学杂志2011，Vol.9Issue(1)：92-96,5.
三种方法转染Math1基因效率和细胞毒性研究
Math1-EGFP gene transfection and cytotoxicity to 293T Cells by different vectors
摘要
Abstract
Objective To determine most effective gene transfection vectors by comparing their transfection efficiency and cytotoxicity to 293T cells. Methods Recombinant adenovirus, lipofectamine 2000 and Superfect nanoparticles were used as gene vectors carrying the Mathl-EGFP gene and pRK5-Mathl-EGFP plasmid, which report gene enhanced green fluorescent protein (EGFP). Expression positive cells were counted under a fluorescence microscope and the survival rate of 293T cells calculated using MTT assay. RT-PCR was used to determine integration of mRNA expression in positive 293T cells. Results By optimizing transfection conditions, the highest transfection efficiency was above 94% for recombinant adenovirus, 73% for Lipofectamine 2000 and 80% for Superfect nanoparticles. The cellular viability was about 90% for Superfect nanoparticles. Conclusion A high transfection efficiency and low cytotoxicity of cells was achieved with Superfect-pRK5- Mathl-EGFP complex nanoparticles, which may be used as a safe and effective vector in research of inner ear diseases.关键词
Math1/基因转染/基因载体/纳米材料Key words
Mathl/ Gene transfer/ Gene vector/ Nanoparticle分类
医药卫生引用本文复制引用
陈志婷,李新新,张小兵,杨仕明,吴南,郭维维,赵立东,刘会占,舒卫宁,任丽丽,王琳..三种方法转染Math1基因效率和细胞毒性研究[J].中华耳科学杂志,2011,9(1):92-96,5.基金项目
国家863计划(2007AA02Z150) （2007AA02Z150）
国家自然科学基金重点项目(30730040),面上项目(30871398,30571017,30000189)和海外青年学者合作基金(30628030) （30730040）
北京市自然科学基金(7042061) （7042061）
国家科技支撵计划重大项目(2007BA118B12,2006BA102B00). （2007BA118B12,2006BA102B00）
