中国造纸学报2011,Vol.26Issue(2):37-41,5.
纤维素酶在不同长度纤维上的吸附行为
Adsorption Behaviors of Cellulase on Cellulose Fiber Fractions with Different Fiber Length
摘要
Abstract
Adsorption of cellulase on the surfaces of cellulose fibers is the first step of cellulose enzymatic hydrolysis and saccharification. The adsorption behaviors of cellulase on cellulose fiber fractions with different fiber length were quantitatively discussed in this study. Hie adsorption equilibrium was reached in around 60 minutes, and the adsorption amount increased with increasing initial cellulase dose. The adsorption process was well described by Langmuir isotherm. The maximum adsorption amount was found on the short fiber, and there was the maximum Langmuir adsorption equilibrium constant in the adsorption on the long fiber, which indicated that cellulase shows the highest adsorption affinity on the long fiber. The calculated thermodynamics parameters indicated that adsorption of cellulase on cellulose fiber is a spontaneous, exothermic and irreversible process. The highest enthalpy change △H° and entropy change △S° were found in the adsorption on 48 mesh fraction and 28 mesh fraction respectively.关键词
纤维素酶/不同长度的纤维/吸附/吸附等温线/热力学常数Key words
cellulase/ different fiber length/ adsorption/ adsorption isotherms/ thermodynamics parameters分类
生物科学引用本文复制引用
吕健,詹怀宇,付时雨..纤维素酶在不同长度纤维上的吸附行为[J].中国造纸学报,2011,26(2):37-41,5.基金项目
本项目得到国家973计划(2007CB210201)和国家建设高水平大学公派联合培养博士研究生项目的资助。 (2007CB210201)