中华耳科学杂志2011，Vol.9Issue(1)：72-76,5.
胆脂瘤囊内碎屑组织萃取物对HaCaT细胞p38MAPK信号通路表达的影响及意义
Effects of cholesteatoma debris extract on expression of p38MAPK signal transduction pathway in keratinocytes and their significance
唐浩程 1张官萍 1李永平 2刘天润 1陆钊群 1袁佛良1
作者信息
- 1. 广州中山大学附属第六医院耳鼻咽喉-头颈外科,广州,510655
- 2. 广州中山大学眼科医院病理科,广州,510060
- 折叠
摘要
Abstract
Objective To build an in vitro model of cholesteatoma epithelium proliferation using HaCaT cells, and to investigate the role of p38 mitogen -activated protein kinase (p38MAPK) signal transduction pathway in the development of cholesteatoma. Methods HaCaT cells were incubated with cholesteatoma debris extract (CDE) to produce an in vitro model of cholesteatoma. Expression of p38MAPK in HaCaT cells was examined by immunohistochemistry and RT-PCR. The impact of SB203580, a p38MAPK inhibitor, on HaCaT cell proliferation was also examined by methyl thiazolyl tetrazolium assay(MTT). Results HaCaT cell proliferation showed does-dependent rise 48 hours later. Phospho-p38MAPK positive staining appeared in the nucleus about 30 min after exposure with CDE, but not in non-exposure specimens. Gel image gray-scale analysis (RT-PCR) readings were 0.216 ±0.027 in the control group, 0.828 ±0.019 in the original CDE exposure group, and 0.S22 ± 0.023 in the 1 : 2 attenuated exposure group. The readings in the two exposure groups were higher than the control group (P < 0.05). MTT assay showed effects of SB20358O on HaCaT cell proliferation following exposure to CDE. The mean OD values in the control group, 2.5 junol/L SB203580group, 5u,mol/L SB203580 group, 10 u,mol/L SB203580 group and 20 u,mol/l SB203580 group were 0.535 ± 0.008, 0.527 ± 0.012, 0.504 ± 0.017, 0.476 ± 0.012 and 0.470 ± 0.013 respectively. There was significant difference between the 5 junol/L, lOjunol/L, 20 jimol/1 exposure groups and control group (P<0.05). Conclusion We believe that p38MAPK is activated in the pathologic process of middle ear cholesteatoma. The p38MAPK signal transduction pathway may play an important role in the proliferation of cholesteatoma epithelium.关键词
胆脂瘤/p38丝裂原活化蛋白激酶/HaCaT细胞/增殖Key words
Cholesteatoma/ p38 mitogen-activated protein kinase (p38MAPK)/ HaCaT cell/ Proliferation分类
医药卫生引用本文复制引用
唐浩程,张官萍,李永平,刘天润,陆钊群,袁佛良..胆脂瘤囊内碎屑组织萃取物对HaCaT细胞p38MAPK信号通路表达的影响及意义[J].中华耳科学杂志,2011,9(1):72-76,5.
