重庆医学2011，Vol.40Issue(31)：3154-3156,3159,4.DOI:10.3969/j.issn.1671-8348.2011.31.014
多西紫杉醇壳聚糖纳米粒制备及其特性研究
Preparation and evaluation of Docetaxel-loaded chitosan nanoparticles in vitro
梁恒伦 1童健 1李晶 2张福伟 1阮宝琴1
作者信息
- 1. 南方医科大学附属珠江医院胸心外科,广州,510282
- 2. 南方医科大学附属珠江医院肿瘤中心,广州,510282
- 折叠
摘要
Abstract
Objective To prepare docetaxel-loaded chitosan nanoparticles(DTX-CTNPs) by optimizing experiment conditions. And the drug-loaded NPs were evaluated for sustain release and in vitro cytotoxicity. Methods DTX-CSNPs were prepared by ion-otropic gelation of chitosan with tripolyphosphate anions (TPP). The NPs were characterized for their shape by scanning electron microscopy. The particle size,size distribution and polydispersity index were assessed by laser scattering. The drug loading,incorporation efficiency and cumulative release rates of the DTX-CTNPs in vitro were analyzed by ultraviolet spectrophotometry. The DTX-CTNPs were evaluated for in vitro cytotoxicity by MTT assay using A54 9 cell lines. Results The DTX-CTNPs(CTS/TPP 5. 3:1. 0) with good shape and narrow size distribution were prepared. The average diameter was 175 nm. The rates of drug loading and encapsulation were (22. 4 + 2. 7)% and (59. 2 + 8. 6)% respectively. And the DTX-CTNPs showed a comparable antitumor efficacy in vitro while strongly reduced the anticancer drug toxicity compared to free DTX (DTX for injection). Conclusion Together our results suggests that the anticancer drug loaded nanoparticles are a promising nano-sized drug vector for cancer therapy.关键词
壳聚糖/纳米球/体外细胞毒性/多西紫杉醇Key words
chitosan/nanospheres/in vitro cytotoxicity/docetaxel引用本文复制引用
梁恒伦,童健,李晶,张福伟,阮宝琴..多西紫杉醇壳聚糖纳米粒制备及其特性研究[J].重庆医学,2011,40(31):3154-3156,3159,4.
