中国药业2011，Vol.20Issue(18)：20-22,3.
尼泊尔菊三七的离体快繁技术研究
Study on Rapid Micropropagation Technique in Vitro of Medical Plant Gynura nepalensis DC.
摘要
Abstract
Objective To establish the method of rapid micropropagation system according to the exploitative situation of Gynura nepalensis DC. Wild resources for getting the best culture conditions. Methods The orthogonal methods were used to determine the optimal concentration of different hormones (6 - BA and NAA) in the differentiation medium with different concentrations of inorganic elements and activated carbon to establish the rapid micropropagation system including multiple shoot clumps induction, rooting, strong seedling and transplanting. Results The sterilizing time of explants in 75% ethanol and 0. 1% mercuric chloride was 20 s and 6 min respectively. The best culture conditions for rapid micropropagation were MS media supplemented with 0. 5 mg/L NAA, 1. 0 mg/L 16 - BA, 30 g/L sucrose and 5.0 g/L agar. The pH value of media was adjusted to 6.0. The optimal media for rooting and strong seedling was as follows: 1/2 MS media supplemented with 0. 5 mg/L NAA, 1. 0 mg/L 6 - BA, 30 g/L sucrose, 2. 0 g/L activated carbon and 5. 0 g/L agar with pH adjusted to 6.0. The best substrates of transplanting were humus and charcoal soil (3 : 2). Conclusion The rapid micopropagation system of Gynura nepalensis DC. Is established successfully, which lays the foundation for large - scale production in future.关键词
尼泊尔菊三七/离体快繁/茎段Key words
Gynura nepalensis DC./rapid micropropagation in vitro/stem segment分类
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高倩,李娜,贾景明..尼泊尔菊三七的离体快繁技术研究[J].中国药业,2011,20(18):20-22,3.
