西南国防医药2011，Vol.21Issue(10)：1064-1067,4.DOI:10.3969/j.issn.1004-0188.2011.10.007
低强度脉冲电磁场对兔动脉粥样硬化斑块中MMP-9及TIMP-1表达的影响
Effects of low-intensity pulse electromagnetic field on expression of MMP-9 and TIMP-1 in atherosclerotic plaques of rabbits
李秀川 1陈易华 2马双陶 2杨大春 3罗艳 3邱力军2
作者信息
- 1. 710032 西安,第四军医大学生物医学工程系
- 2. 成都军区总医院病理科
- 3. 成都军区总医院心血管内科
- 折叠
摘要
Abstract
Objective To investigate the effects of low - intensity pulse electromagnetic field( LPEF )on the expression of matrix metalloproteinase - 9( MMP - 9 )and tissue inhibitor of metalloproteinase - l( TIMP - 1 )in atherosclerotic( AS )plaques of rabbits. Methods Rabbits were randomly divided into three groups: normal diet( ND )group, high fat diet( HD )group and HD plus magnetic field( HM )group. The ND and the HD group were respectively given regular diet and high fat diet. The HM group was given high fat diet, and meanwhile received intervention of LPEF. After intervention for 7 w,the aortic roots of these animals were dissected for routine paraffin sections and HE staining,and the area of AS plaque was calculated. In addition,the expression of MMP - 9 and TIMP -1 in AS plaque was detected by immunohistochemical staining. Results HD treatment significantly increased the area of AS plaque in aortic root compared with ND treatment P < 0. 05 ). LPEF intervention significantly attenuated HD - induced formation of AS plague ( P < 0. 05 ). HD treatment significantly elevated the expression of MMP - 9 and TIMP - 1 in AS plaque( P < 0. 05 ). However, the intervention of LPEF significantly inhibited the HD - induced increases of the expression level of MMP - 9 and TIMP - l( P < 0. 05 ), and remarkably decreased the ratio of MMP - 9 to TIMP - 1. Conclusion The anti - AS mechanism of LPEF treatment may be related to decrease the ratio of MMP - 9 to TIMP - 1.关键词
动脉粥样硬化/低强度脉冲电磁场/MMP-9/TIMP-1Key words
atherosclerosis/low - intensity pulse electromagnetic field/MMP - 9/TIMP - 1分类
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李秀川,陈易华,马双陶,杨大春,罗艳,邱力军..低强度脉冲电磁场对兔动脉粥样硬化斑块中MMP-9及TIMP-1表达的影响[J].西南国防医药,2011,21(10):1064-1067,4.
