中国药业2011，Vol.20Issue(18)：30-31,2.
反相高效液相色谱法测定茵陈合血胶囊中黄芩苷含量
Determination of Baicalin in Yinchen Hexue Capsule by RP——HPLC
摘要
Abstract
Objective To establish a HPLC method for the determination of baicalin in Yinchen Hexue Capsule. Methods The separation of sample was carried on the Shimpak VP - ODS column(250 mm×4. 6 mm, 5μm) with the mobile phase of methanol - water - acetic acid (52 :48 :1) at the flow rate of 1.0 mL/min. The detection wavelength was set at 280 nm. The sample size was 10μL and the column temperature was 25 t. Results The chromatographic peaks of baicalin and impurities were separated well. The mean recovery rate of baicalin was 99.96%, RSD = 0. 23% (n = 6). The inter -day RSD and intra - day RSD were all less than 5%. The linearity was determined over the range of 10. 55 -63. 30 mg/L( r =0. 999 8). Conclusion This method is simple, sensitive, rapid, accurate and reproducible, which is suitable for the quantitative determination and the stability study of baicalin in Yinchen Hexue Capsule.关键词
茵陈合血胶囊/黄芩苷/反相高效液相色谱法Key words
Yinchen Hexue Capsule/baicalin/RP - HPLC分类
医药卫生引用本文复制引用
蒋大义,张娓,杨宏图,马惠莱,王惠玲,常翠..反相高效液相色谱法测定茵陈合血胶囊中黄芩苷含量[J].中国药业,2011,20(18):30-31,2.基金项目
深圳市科技计划项目,项目编号:200903011 （）
广东省医院药学研究基金项目,项目编号:2011A30. （）
