国际医药卫生导报2011,Vol.17Issue(18):2280-2282,3.DOI:10.3760/cma.j.issn.1007-1245.2011.18.029
手工制作组织芯片的技术心得
The technology and effect of handmade tissue microarray
黎晴 1杨梅 2曹桂明2
作者信息
- 1. 510170 广州市荔湾区人民医院病理室
- 2. 421000 湖南省衡阳市南华大学附属南华医院病理科
- 折叠
摘要
Abstract
Objective To introduce a technology of manual manufacturing tissue microarray.Methods The improved needle, coverslip plastic packaging box and X ray film were used to produce hand-made tissue microarray, and then conducted with immunohistochemistry (IHC) and HE staining.Results A complete array of wax, neat and orderly core of tissue microarray were found, without any shift,and with satisfactory staining of tissue microarray sections.Conclusion The method of hand-made preparation of tissue microarray is simple with less cost, and the quality could be matching that of the imported tissue microarray instrument or tissue chip made by machine producer, easy to be spreaded.关键词
组织芯片;HE染色;免疫组化染色Key words
Tissue microarray/HE staining/immunohistochemistry (IHC)引用本文复制引用
黎晴,杨梅,曹桂明..手工制作组织芯片的技术心得[J].国际医药卫生导报,2011,17(18):2280-2282,3.