中山大学学报（医学科学版）2011，Vol.32Issue(3)：321-326,6.
蛋白酶体抑制剂MG132对Raji细胞凋亡、细胞周期及UBE1基因表达的影响
Effects of Proteasome Inhibitor MG132 on Apoptosis, Cell Cycle Distribution, and Expression of Ubiquitin-activing Enzyme 1 (UBE1) in Raji Cells
摘要
Abstract
[Objective] To explore the effects of proteasome inhibitor MG132 on apoptosis,cell cycle distribution,and the expression of ubiquitin-activing enzyme 1 (UBE1) in human Burkitt's lymphoma Raji cells. [Methods] Raji cells were cultured and treated with 5 |xmol/L MG132 for 24 h, morphological changes of cells were observed under inverted microscope. Apoptosis and cell cycle distribution were assessed by flow cytometry. Expression of UBE1 mRNA and protein were determined by real-time RT-PCR and Western blot assay. [Results] Raji cells grew slowly after exposure to MG132, dead and apoptotic cells could be seen. The apoptosis rate in MG132 group was much higher than that in control group [ (26.06 ± 1.10)% vs (0.30 ± 0.26)%, P < 0.05] and the cells in G2/M phases with MG132 treatment were significantly higher than those in control group [ (33.2 ± 1.2)% vs ( 12.5 ±1.1)% ,P < 0.05]. The mRNA expression of UBE1 in MG132 group remarkably reduced compared to the control(0.35 ± 0.05 vs 1.00 ± 0.08,P< 0.05) with inhibition ratio of 65% and UBE1 protein was also down-regulated by MG132 (0.93±0.07 vs 1.62±0.22, P<0.05) with the protein expression ratio of (58±6)%. [Conclusion] MG132 can induce apoptosis and G2/M arrest of Raji cells, it can also inhibit the expression of UBE1 mRNA and protein of Raji cells significantly, the mechanisms involved needs further investigation.关键词
泛素-蛋白酶体途径/蛋白酶体抑制剂/淋巴瘤/Raji细胞Key words
ubiquitin-proteasome pathway/ proteasome inhibitor/ lymphoma/ Raji cells分类
生物科学引用本文复制引用
刘勇,龙捷,莫祥兰,苏祖兰,邵春奎,谢晓斌..蛋白酶体抑制剂MG132对Raji细胞凋亡、细胞周期及UBE1基因表达的影响[J].中山大学学报（医学科学版）,2011,32(3):321-326,6.基金项目
广东高校优秀青年创新人才培育项目(LYM08084)，广东省医学科研基金(B2010141) （LYM08084）
