陕西师范大学学报（自然科学版）2011，Vol.39Issue(3)：56-61,6.
丹参铜锌超氧化物歧化酶(Cu/Zn-SOD)基因的克隆与生物信息学分析
Cloning and bioinformatics analysis of Cu/Zn-SOD gene from Salvia miltiorrhiza Bunge
摘要
Abstract
Based on the constructed cDNA library* a Cu/Zn-superoxide dismutase gene (Cu/Zn-SOD) from Salvia miltiorrhiza Bunge was cloned by TD PCR. The characteristics including the composition of amino acid sequences, hydrophobicity or hydrophilicity, secondary structure of protein and function of the Cu/Zn-SOD were analyzed and predicted by the tools of bioinformatics. The results showed that SmCu/Zn-SOD is a hydrophilic, non-transmembrane and non-secretory protein. The main motif of Cu/Zn-SOD in secondary structure was random coils and extended strands. Despite sequence variations, the core structure, sequence and activity sites of SmCu/Zn-SOD were highly similar to that of other green plants.关键词
Cu/Zn-SOD/降落PCR/丹参/生物信息学Key words
Cu/Zn-SOD/ TD PCR/ Salvia miltiorrhiza Bunge/ bioinformatics分类
农业科技引用本文复制引用
纪砚耘,化文平,王喆之..丹参铜锌超氧化物歧化酶(Cu/Zn-SOD)基因的克隆与生物信息学分析[J].陕西师范大学学报（自然科学版）,2011,39(3):56-61,6.基金项目
"十一五"科技支撑计划项目(2006BAI06A12) （2006BAI06A12）
