陕西师范大学学报（自然科学版）2011，Vol.39Issue(3)：62-66,5.
秦岭山区中国林蛙致病菌的分离鉴定
Isolation and identification of the pathogens from Rana chensinensis in Qinling Mountains
摘要
Abstract
The Rana chensinensis widely distributed in Qinling Mountains were liable to be infected in Dayukou (region A, 109°6'52"E, 34°0'56"N) than Shibianyu (region B, 1O8"56'33"E, 34°0'20"N). Six microorganisms were isolated from the infected Rana chensinensis at region A, among which, Aeromonas hydrophila is a strong pathogen to Rana chensinensis according to the data from artificial infections. The bacteria-community structures of two regions were analyzed by PCR-DGGE (PCR-denaturing gradient gel electrophoresis), and the quality of water was examined. The results showed that a rich bacteria diversity was found in the soil and water from both region A and B, but there were significant differences between A and B. Meanwhile, the content of six strains isolated from Rana chensinensis in A and B was different, and the population of Aeromonas hydrophila in region A was richer than that of region B. Particularly, several strains highly homologous with the stains identified from sewerage were isolated at region A. The water quality analysis indicated that the water quality of region A is lower than that of region B. So, the deterioration of environment at region A was assumed to be the major causation for the infection of the wild Rana chensinensis.关键词
中国林蛙/致病菌/变性梯度凝胶电泳/细菌群落Key words
Rana chensinensis j pathogen? PCR-denaturing gradient gel electrophoresis5 bacteria-community分类
农业科技引用本文复制引用
孟岳峰,李治,孙燕,赵洁,张红娟..秦岭山区中国林蛙致病菌的分离鉴定[J].陕西师范大学学报（自然科学版）,2011,39(3):62-66,5.基金项目
陕西省自然科学基础研究计划项目(SJ08-ZT08 （）
2009JQ2003) （）
陕西师范大学实验室开放基金资助项目. （）
