中国中西医结合外科杂志2011，Vol.17Issue(5)：489-491,3.DOI:10.3969/j.issn.1007-6948.2011.05.013
大鼠肺泡Ⅱ型上皮细胞的分离、纯化和鉴定
Isolation, Puritication and Identification of Alveolar Type II cells of Rats
摘要
Abstract
Objective To study the method of primary culture of alveolar type Ⅱ (AT Ⅱ) cells. Methods AT Ⅱ cells were isolated by Dobbs's method. The cells were purified by using rat IgG coated dishes and were characterized by using electronic microscopy and SP-A immunohistochemical staining. The cellular ultra-structure of AT Ⅱ cells was observed by electronic microscopy. Results The trypan blue staining showed the cell viability over 95 percent. Immunohistochemical staining using anti-SP - A demonstrated that the purity of AT Ⅱ cells was 92%. Conclusion In AT Ⅱ cells isolation, purification and identification procedures, optimizing the concentration of digest enzyme and time can improve the cell vitality. Purification of AT Ⅱ cells with IgG coated plate is a better method than other reported methods. AT Ⅱ cells could be characterized through detection of the lamellar bodies with electronic microscopy and SP-A Immunohistochemical staining.关键词
肺泡Ⅱ型上皮细胞/肺表面活性物质相关蛋白A/细胞培养Key words
Alveolar type Ⅱ cells/ Surfactant protein A/ Cell culture分类
生物科学引用本文复制引用
张雪梅,陈海龙,王朝晖,张利,纪军..大鼠肺泡Ⅱ型上皮细胞的分离、纯化和鉴定[J].中国中西医结合外科杂志,2011,17(5):489-491,3.基金项目
国家自然科学基金资助项目(30973851) （30973851）
大连市卫生局2010年科研基金资助项目 （）
