中南大学学报（自然科学版）2011，Vol.42Issue(6)：1560-1566,7.
酶制剂在玉米秸秆双菌种固态发酵生产微生物油脂中的应用
Application of enzyme in production of microbial oils fermented by two-strains with corn stalk
摘要
Abstract
The corn stalk was treated with cellulase and Trichoderma koningii to produce reducing sugar, then the production was re-fermented by Trichosporon cutaneum to produce microbial oils. The best process conditions were obtained by orthogonal test through measuring the content of reducing sugar and microbial oils in product. The results show that the reducing sugar content of the corn stalk treated with cellulase and Trichoderma koningii is 4.852 g/L under the conditions that the additive of cellulase is 0.15%, and the additive of Trichoderma koningii is 10% at 26 ℃ for 6 d. The microbial oil content of the corn stalk re-fermented by Trichosporon cutaneum is 3.321 g per 100 g fermentation products under the condition that the additive of Trichosporon cutaneum is 9 % at 26 ℃ for 7 d.关键词
玉米秸秆/纤维素酶/康宁木霉/皮状丝孢酵母/发酵/微生物油脂Key words
corn stalk/ cellulase/ Trichoderma koningii/ Trichosporon cutaneum/ fermentation/ microbial oil分类
生物科学引用本文复制引用
李新社,陆步诗,金双喜..酶制剂在玉米秸秆双菌种固态发酵生产微生物油脂中的应用[J].中南大学学报（自然科学版）,2011,42(6):1560-1566,7.基金项目
湖南省科技厅资助科研项目(2010JT4055) （2010JT4055）
