重庆医学2011，Vol.40Issue(29)：2947-2948,2951,3.DOI:10.3969/j.issn.1671-8348.2011.29.013
负载miR-138复制缺陷型腺病毒对hTERT表达的影响
Effects of replication-deficient adenovirus carrying miR-138 on the hTERT expression
摘要
Abstract
Objective To identify effects of the replication-deficient recombinant adenovirus carrying miR-138 on the expression level miR-138 and the target gene hTERT. Methods The human gastric cancer cell line BGC-823 were infected with the replication-deficient recombinant adenovirus carrying miR-138 by the MOI of 200 : 1. The expression levels of miR-138 and hTERT mRNA were inuestigated by real-time PCR. The expression of hTERT protein was detected by western blot. Results Compared to the negative control, after infecting the replication-deficient recombinant adenovirus carrying miR-138 , BGC-823 cells expressed higher level of miR-138 (9. 152 ± 2. 641 vs. 0. 928 ± 0. 199,P<0. 05). No significant difference was observed between the ZERO group (BGC-823 infecting no adenovirus) and negative control (1. 085 ± 0. 478 vs. 0. 928 ± 0. 199,P>0. 05). The expression of hTERT protein was down-regulated in the BGC-823 cells after infected adenovirus carrying miR-138. Conclusion The replication-deficient recombinant adenovirus carrying miR-138 can up-regulate the expression of miR-138 effectively and suppress the expression of hTERT protein.关键词
RNA指导的DNA聚合酶/胃肿瘤/复制缺陷型腺病毒载体Key words
RNA-directed DNA polymerase/ stomach neoplasms/ replication-deficient adenoviral vector引用本文复制引用
刘志鹏,王宗华,梁光萍,熊晓峰,范亚川,邹利全,王洪斌,陈陵,李学成..负载miR-138复制缺陷型腺病毒对hTERT表达的影响[J].重庆医学,2011,40(29):2947-2948,2951,3.基金项目
重庆市自然科学基金资助(CSTC2008BB5274,CSTC2009BB5314,CSTC2010BB5158). （CSTC2008BB5274,CSTC2009BB5314,CSTC2010BB5158）
