安徽医科大学学报2011，Vol.46Issue(10)：1013-1017,5.
橙皮苷对大鼠胶原性关节炎的治疗作用及部分机制
Therapeutical effect of hesperidin on collagen-induced arthritis in rats and its mechanisms
摘要
Abstract
To study the therapeutic effect of hesperidin ( HDN ) on collagen-induced arthritis( CIA ) in rats and its mechanisms. Methods CIA was induced with collagen II and Freund' s complete adjuvant. The effect of HDN on CIA was evaluated by hind paw swelling and histopathologica changes. Interleukin-17( IL-17 ) in serum was examined by enzyme linked immunosorbent assay ( ELISA ). Splenic lymphocyte proliferation response induced by concanavalin A ( conA ) and lipopolysaccharide ( LPS ) was examined with MTT assay. The lever of phagocytosis of ( PMΦ ) was measured by neutral red method. Interleukin-1 ( IL-1 ) and tumor necrosis factor-α ( TNF-α ) in PMΦ of CIA rats were measured by ELISA. Results HDN( 80,160,320 mg/kg ) singnificantly inhibited the paw swelling of CIA rats. The pathological damage of knee joint was reversed by HDN. The level of IL-17 in serum was decresed by HDN( 160,320 mg/kg ). The enhanced lymphocyte proliferation in CIA rats was reversed by treatment with HDN. HDN( 10-6 ,10-5,10-4 mol/L )singnificantly reduced the phagocytosis of neutral. Meanwhile, HDN ( 10-4 mol/L) markedly reduced IL-1 and HDN ( 10-6 ,10-5,10-4 mol/L ) markedly reduced TNF-a in CIA rats. Conclusion HDN has marked therapeutic effection on CIA rats,which is related to reducing the phagocytosis and inhibiting the secretion of pro-inflammatory cytokines.关键词
橙皮苷/药理学/关节炎/免疫调节Key words
hesperidin/pharmacology/ arthritis/ immunity分类
医药卫生引用本文复制引用
熊立,李俊,王雯,楼蓉,凡小燕,黄艳..橙皮苷对大鼠胶原性关节炎的治疗作用及部分机制[J].安徽医科大学学报,2011,46(10):1013-1017,5.基金项目
国家重大基础研究前期(973前期)研究专项(编号:2002CCC02900) （973前期）
