摘要
Abstract
Objective To explore the relationship between the number of secondary polar body (PB) and the developing potential of embryos. Methods According to the number of PB within oocytes after short-term fertilization, four groups were divided; 1-PB group, 2-PB group, 3-PB group and > 3-PB group. Several items were observed; normal/abnormal fertilization rate (2PN, 1PN and 3PN contained respectively) , top quality embryos rate, percentage of transferred embryos and implantation rate of each group. Chi-square test was used. Results CD The 2PN rate in 2-PB group was significantly higher than other groups, while the abnormal fertilization rate in 2-PB group was the lowest among the four groups. With the increase of secondary PB number, the abnormal fertilization rate was increased accordingly; (2) The embryo implantation rate in 2-PB and 3-PB groups was remarkably higher than 1-PB group; (3) There was no significant difference in the transferred embryo rates between 2PB and 3PB groups. Conclusion The oocytes with 2 PB after short-term fertilization are related to the rate of total fertilization, normal fertilization, top quality embryo, embryo utilization and implantation, inferring a high developing potential of these embryos. Furthermore, along with the increase of secondary PB number ( > 3 PB) after short-term fertilization, the abnormal fertilization rate is increased, which may be related to the abnormal mei-osis or gene regulation. These embryos are not recommended to be transferred firstly. In addition, considering the total fertilization rate of 1PB group is up to 48. 9% , it is necessary to prolong the o-ocyte-observed time after short-term fertilization, so rescue ICSI could be avoidedoverusing. Finally, the embryo implantation rate in 1-PB group is significantly lower than 2-PB and 3-PB group, so the embryos from 1-PB group are also not recommended to be transferred firstly. Considering all together, oocytes with 2-PB after short-term fertilization possess high developing potential, and observing the number of secondary PB after short-term fertilization may be a useful supplement to evaluate embryos developing potential.关键词
第二极体/受精/胚胎质量/着床率/发育潜能Key words
secondary polar body/ fertility rate/ embryonic quality/ implantation rate/ developing potential分类
医药卫生
