中国组织工程研究与临床康复2011，Vol.15Issue(32)：5896-5900,5.DOI:10.3969/j.issn.1673-8225.2011.32.002
人骨髓间充质干细胞分离培养方法的改进
Improvement of methods to isolate and culture human mesenchymal stem cells
摘要
Abstract
BACKGROUND: The methods to isolate mesenchymal stem cells (MSCs) can be classified to be several kinds. However, themethod of acquisition in surgery is still less. The isolated MSCs from the patients who receive the replacement operation can offersome data which may reveal the causes or clues of some diseases.OBJECTIVE: To isolate and culture MSCs with an improved technique of differential anchoring velocity and adherence, in order toidentify MSCs.METHODS: The bone marrow blood was collected in the femur marrow cavity duiring the hip joint replacement. MSCs wereisolated by adherence method in the special medium and the mix medium was flushed 36-48hoursaftertheinoculationtoisolateMSCs from the marrow mixture. In the long-time culture in vitro, the special medium was used to weed other cells out. Theanchored cell was cultured and identified when the amount was large enough.RESULTS AND CONCLUSION: MSCs can be isolated from the bone marrow in long cavitas medullaris, and can be cultured intocolonies by using adherence method. The morphological features and surface molecules are identified consistent with MSCs, andcan obtain standard MSCs, so as to do the follow-up experiment.关键词
人骨髓间充质干细胞/细胞培养/鉴定/流式细胞术/干细胞分离与培养分类
基础医学引用本文复制引用
王承云,石磊,夏春,郑欣鹏,张兵,林飞太,王少杰..人骨髓间充质干细胞分离培养方法的改进[J].中国组织工程研究与临床康复,2011,15(32):5896-5900,5.基金项目
2009厦门科技基金项目课题子课题(3502Z20094008),课题名称:股骨头坏死间充质干细胞成骨与凋亡过程中NF-kappaB调控caspase-3的研究. （3502Z20094008）
