中国组织工程研究与临床康复2011，Vol.15Issue(32)：5901-5904,4.DOI:10.3969/j.issn.1673-8225.2011.32.003
胚胎骨髓来源间充质干细胞对人Th17细胞的免疫调节
Immunoregulatory function of fetal bone marrow-derived mesenchymal stem cells on human Th17 cells
摘要
Abstract
BACKGROUND:Many studies have demonstrated mesenchymal stem cell(NSC) can regulate the immune fuction and inhibit proliferation of T cell.OBJECTIVE:To investigate the regulatory function of fetal bone marrow derived mesenchymal stem cells (FBM-MSCs) on human Th17 cells.METHODS:FBM-MSCs were cocultured with human peripheral blood mononuclear cells(FBMCs) or CD4+ T cells at 1∶10 ratio from healthy donors for 4 days.Single culture of monouclear cells or CD4+ T cells were as controls.The expression of interleukin-17(IL-17) mRNA was detected by real-time PCR,IL-17 protein was assaed by enzyme-labeled innunosorbent assay(ELISA),and quantity of Th17 cells was observed by flow cytometry.RESULTS AND CONCLUSION:The level of IL-17mRNA in FBM-MSC cocultured with PBMC(FBM-MSC/PBMC)group was significantly higher than that in PBMC cultured alone.Meanwhile,IL-17 expression in supematant in FBM-MSCPBMC/CD4+ was significantyl higher than that in PBMC and CD4+T cells groups(P＜0.05,P＜0.01).The number of FBM-MSCs/CD4+ T cells group was significantly more than CD4+ T cells group(P＜0.01).However,FBM-MSCs did not express IL-17 protein.FBM-MSCs can promote the proliferation of Th17 cells.关键词
胚胎/骨髓/间充质干细胞/Th17细胞/免疫调节分类
基础医学引用本文复制引用
郭振兴,郑翠玲,陈振萍,董文川,杨仁池..胚胎骨髓来源间充质干细胞对人Th17细胞的免疫调节[J].中国组织工程研究与临床康复,2011,15(32):5901-5904,4.基金项目
本课题由国家自然科学基金(81000228)资助. （81000228）
