中国组织工程研究与临床康复2011，Vol.15Issue(32)：5905-5908,4.DOI:10.3969/j.issn.1673-8225.2011.32.004
DNA去甲基化对大鼠骨髓间充质干细胞增殖细胞核抗原的调节
Effect of DNA demethylation on proliferating cell nuclear antigen expression in bone marrow mesenchymal stem cells
赵宏贤 1徐富翠 1王巧稚 1徐炝 1陈霞2
作者信息
- 1. 泸州医学院组胚教研室,四川省泸州市,646000
- 2. 泸州医学院附属医院消化内科,四川省泸州市,646000
- 折叠
摘要
Abstract
OBJECTIVE: To explore the effect of DNA demethylation on proliferation and proliferating cell nuclear antigen (PCNA) protein expression in bone marrow mesenchymal stem cells. METHODS: Bone marrow mesenchymal stem cells were isolated by using the method of adhesive culture of whole bone marrow. In the third passage bone marrow mesenchymal stem cells, CD44, CD45 and PCNA protein were dectcted by immunocytochemistry, and effect of DNA demethylation on proliferation was detected by MTT method. RESULTS AND CONCLUSION: ①The third passage bone marrow mesenchymal stem cells expressed CD44 postively and CD45 negatively. ②Compared with control group, after 48-hour intervention of 5-azacytidine, 5-azacytidine with 6, 12, 24 μmol/L increased proliferation of cells without concentration-dependence significantly (P ＜ 0.05). Compared with control ③group, after 48-hour intervention of 5-azacytidine, 5-azacytidine with 6, 12, 24 μmol/L increased PCNA protein expression significantly in bone marrow mesenchymal stem cells without concentration-dependence (P ＜ 0.05). In certain concerntration, 5-azacytidine can increase PCNA protein expression thirough DNA demethylation, which is the key to improve proliferation of bone marrow mesenchymal stem cells.关键词
增殖细胞核抗原/骨髓间充质干细胞/DNA去甲基化/5-杂氮胞苷/增殖分类
医药卫生引用本文复制引用
赵宏贤,徐富翠,王巧稚,徐炝,陈霞..DNA去甲基化对大鼠骨髓间充质干细胞增殖细胞核抗原的调节[J].中国组织工程研究与临床康复,2011,15(32):5905-5908,4.
