中国组织工程研究与临床康复2011，Vol.15Issue(32)：5932-5935,4.DOI:10.3969/j.issn.1673-8225.2011.32.010
脂肪基质干细胞和骨髓基质干细胞的体外分化能力
In vitro differentiation capacity of adipose-derived stromal stem cells and bone marrow stromal stem cells
摘要
Abstract
BACKGROUND: Adipose-derived stromal stem cells (ADSCs) have many biological properties similar to bone marrow stromal stem cells (BMSCs). OBJECTIVE: To compare the directed differentiation of ADSCs and BMSCs co-cultured with damaged PC12 cells. METHODS: Stromal stem cells were isolated from adipose tissue and bone marrow. The fifth passage cells were selected. Two kinds of cells were co-cultured, respectively, with or without normal or damaged PC12 cells supernatant. RESULTS AND CONCLUSION: CD44 and CD29 highly expressed in ADSCs and BMSCs, while CD45 and CD56 were only detected in BMSCs, but rare in ADSCs. After single culture, two kinds of cells highly expressed Nanog, Oct4, Sox2, but not neuron-specific enolase (NSE). The levels of Nanog, Oct4, Sox2 in the cells co-cultured with damaged PC12 cells were significantly lowered, while NSE positive cells were increased in ADSCs. These indicate that damaged PC12 cells may have a stronger effect on induce and differentiation of ADSCs.关键词
脂肪基质干细胞/骨髓基质干细胞/共培养/体外分化/神经变性疾病分类
医药卫生引用本文复制引用
周进,田国萍,王静娥,徐冰,李莉,朱峰,韩建,胡凤杰..脂肪基质干细胞和骨髓基质干细胞的体外分化能力[J].中国组织工程研究与临床康复,2011,15(32):5932-5935,4.基金项目
2009沈阳市科委基金项目课题(1091161-0-00):骨髓基质干细胞与脂肪基质干细胞生物学特性对比的研究. （1091161-0-00）
