中国组织工程研究与临床康复2011，Vol.15Issue(32)：5967-5970,4.DOI:10.3969/j.issn.1673-8225.2011.32.017
骨形态发生蛋白2对14d胎鼠端脑神经干细胞分化为胆碱能神经元的影响
Effects of bone morphogenetic proteins 2 on neural stem cells of 14-day-old fetal rat telencephalon differentiating into cholinergic neurons
摘要
Abstract
BACKGROUND: Bone morphogenetic protein-2 (BMP2) may be an extracellular regulatory factor involved in cholinergic differentiation of neuronal precursor cells. OBJECTIVE: To explore the effects of BMP2 on neural stem cells of 14-day-old fetus rat telencephalons induced into cholinergic neurons. METHODS: Fetus telencephalons of 14-day-old SD rats were isolated, and tissues were divisively treated with collagenase typeⅠcontained EDTA following trituration. The cells were raised in polylysine culture plates with serum-free medium. Primary culture medium was changed half after 24 hours, and 10 μg/L BMP2 was added. RESULTS AND CONCLUSION: The adherent monoculture neural stem cells could been obtained using collagenase. The Nestin positve cells were obtained, and the purity was over 99%. After induced by BMP2, the ChAT positve cells were obtained, and the purity was over 97%. BMP2 can induce neural stem cells of 14-day-old fetus rat telencephalons into cholinergic neurons.关键词
骨形态发生蛋白2/神经干细胞/胆碱能神经元/端脑/胎鼠分类
医药卫生引用本文复制引用
梁军,李明秋,赵富生,董建将,李月珍..骨形态发生蛋白2对14d胎鼠端脑神经干细胞分化为胆碱能神经元的影响[J].中国组织工程研究与临床康复,2011,15(32):5967-5970,4.基金项目
牡丹江市科学技术计划项目(G2010s0020),课题名称"基底前脑胆碱能神经元发生的研究". （G2010s0020）
