中国医学科学院学报2011，Vol.33Issue(4)：408-411,4.DOI:10.3881/j.issn.1000-503X.2011.04.012
雌激素制备大鼠子宫肌瘤动物模型
Estrogen-induced Rat Model of Uterine Leiomyoma
陈海刚 1朱兰 1崔全才 2郎景和 1李玢1
作者信息
- 1. 中国医学科学院北京协和医学院北京协和医院妇产科 北京100730
- 2. 中国医学科学院北京协和医学院北京协和医院病理科 北京100730
- 折叠
摘要
Abstract
Objective To establish an appropriate animal model of uterine leiomyoma and to understand the pathogenesis of this disease. Methods Mature female rats were intramuscularly injected with estradi-ol benzoate at 200 μg or 300 μg twice a week. After injection for 8 or 10 weeks, the rats were sacrificed. We measured the serum levels of estrogen (E2) and progesterone (P) , evaluated ER and PR expression, and calculated the leiomyoma forming rate and mortality of the rats. Histological changes were compared between rat u-terine leiomyoma and human uterine leiomyoma with HE staining. The optimal dose and duration of E2 for induction of uterine leiomyoma in rat were determined. Results In the rats treated with estradiol benzoate 200 μg for 8 weeks, the serum E2 level increased significantly (P <0. 01 ). Uterine nodules were visible in some of the tested rats. Based on the pathohistological results, the uterine leiomyoma developed in the treated rats demonstrated similar features as in human uterine leiomyoma. The expressions of ER and PR were increased in the leiomyoma tissues. Conclusions The rat model of uterine leiomyoma can be established by intramuscular injection of estradiol benzoate at 200 μg twice per week for 8 weeks, with similar features as those of human uterine leiomyoma. The high concentrations of ER and PR in uterine tissue might be related with the development of uterine leiomyoma in animal.关键词
子宫肌瘤/动物模型/大鼠/雌激素Key words
uterine leiomyoma/ animal model/ rat/ estrogen分类
医药卫生引用本文复制引用
陈海刚,朱兰,崔全才,郎景和,李玢..雌激素制备大鼠子宫肌瘤动物模型[J].中国医学科学院学报,2011,33(4):408-411,4.
