广西植物2011，Vol.31Issue(4)：545-549,5.DOI:10.3969/j.issn.1000-3142.2011.04.024
水稻花药愈伤组织诱导的多因子正交试验研究
Studies on anther callus induction of rice by orthogonal test
王辉 1肖小君 1齐泽民 1黄作喜 1王芳1
作者信息
- 1. 内江师范学院生命科学学院,四川省高等学校特色农业资源研究与利用重点实验室,四川内江641112
- 折叠
摘要
Abstract
Using the anther of Milyang 46 and the F1 hybrids of Pei'ai 64/8006.the correlation of pollen development with the distance of the pulvinus was studied. The results showed that most of the microspores were in the late uni-nucleate stage when the distance of the pulvinus was 5-7 cm for Milyang 46; while it was 7-9 cm for the F1 hybrids of Pei'ai 64/8006. Besides,the effects on anther callus induction rate of the types of medium,the ration of plant hormones, the concentration of the sucrose and 2,4-D were investigated by using orthogonal tests L9(34),and the best conditions for callus induction of the two accessions were selected: the Milyang 46 was SK3 + 4 mg · L-1 2,4-D+1 mg· L-1 NAA+0. 5 mg · L-1 KT+3%sucrose while the Fl hybrids of Pei'ai 64/8006 was HE5+2 mg · L-1 2,4-D + 1 mg · L-1 NAA+0. 5 mg · L-1 KT +6%sucrose.关键词
水稻/花药/愈伤组织诱导/正交试验Key words
rice/ anther/ callus induction/ orthogonal test分类
生物科学引用本文复制引用
王辉,肖小君,齐泽民,黄作喜,王芳..水稻花药愈伤组织诱导的多因子正交试验研究[J].广西植物,2011,31(4):545-549,5.
