重庆医学2011，Vol.40Issue(29)：2949-2951,封3,4.DOI:10.3969/j.issn.1671-8348.2011.29.014
蛋白酶体抑制剂诱导大鼠帕金森病模型的建立
Establish of Parkinsons's disease model induced by lactacystin in rats
汪纯 1马石楠 2杨棋 2王洋 2戢翰升1
作者信息
- 1. 湖北医药学院附属十堰市人民医院神经外科,442000
- 2. 湖北医药学院附属太和医院神经外科,湖北十堰,442000
- 折叠
摘要
Abstract
Objective To establish one model of Parkinson's disease(PD)for rats, and to comprehensively evaluate the model. Methods The rat PD model was established by the unilateral single-path and two-targets injection of lactacystin into the NSr through stereotaxic technique. And then,the behaviors features,the tyrosine hydroxylase-immunoreactivity in substantia nigra were observed. Results After lactacystin injection for 1 week, the behavior of rats was changed, including progressively bradykinetic, contralateral head tilting and tremor,and apomorphine-induced contralateral behavior was observed. After 4 weeks,sports activity of rats in each group were reduced compared with the saline group. Pathological examination revealed microglia proliferation and do-paminergic neurons swelling or shrinking in the needle parts;The change in 8 μg group was more apparent,and some of nigral cells in it contained Lewy body. The number of tyrosine hydroxylase positive cells in 2 μg, 4 μg and 8 μg groups were significantly de-creased(P<0. 01) compared with the control group. And as the dose increased the number of TH immunoreactive cells gradually decreased. Conclusion The rat PD model established by the unilateral single-path and two-targets injection of lactacystin into the NSr through stereotaxic technique is similar to the clinical PD both in symptoms and pathological changes.关键词
帕金森病/大鼠/蛋白酶体抑制剂Key words
Parkinson disease/rats/lactacystin引用本文复制引用
汪纯,马石楠,杨棋,王洋,戢翰升..蛋白酶体抑制剂诱导大鼠帕金森病模型的建立[J].重庆医学,2011,40(29):2949-2951,封3,4.
