重庆医学2011，Vol.40Issue(29)：2952-2954,2958,封3,封4,6.DOI:10.3969/j.issn.1671-8348.2011.29.015
银杏叶提取物对大鼠重症急性胰腺炎肾损伤的保护作用及机制研究
The study on the protective effect and mechanism of ginkgo extract to the kidney injury of rats with SAP
何彩 1王煊 1邓明明 1周正端 1刘宏 1屈小平1
作者信息
- 1. 泸州医学院附属医院消化内科,四川泸州,646000
- 折叠
摘要
Abstract
Objective To study the protective effect and the possible mechanism of ginkgo extract on the kidney injury of rats with SAP. Methods 90 female Sprague-Dawley rats were randomly divided into sham operation group(N group) , severe acute pancreatitis group(P group) extractive of ginkgo treatment group(S group) respectively. 5% sodium taurocholate was retrogradely injected into the pancreatic duct of rats in P and S groups to induce SAP. Rats of every group were respectively and humanely killed at 12 h,24 h,48 h to compare the kidney injury in every group for general morphology and light microscopy. Blood was obtained for BUN,Cr. TNF-αand IL-6 level detecting by ELISA,TLR4 and NF-κB in the kidney tissues were semi-quantified detected by immu-nohistochemical method. All the results were analysed by Anova with SPSS16. 0. Results The injury of kidney tissues in P group was obvious,pathological scores were higher than those in N group. So were the concentrations of BUN、Cr、TNF-αand IL-6 and the expressions of TLR4 and NF-κB in the kidney tissues. All the results in S group were lower than those in P group. Conclusion The extraction of ginkgo has satisfactory therapeutic effect on kidney injury of rats with SAP depending on the activation inhibition of TLR4 and NF-κB.关键词
胰腺炎/Toll样受体4/NF-κB/银杏叶提取物Key words
pancreatitis/Toll-like receptors/ NF-kapaa B/extractive of ginkgo引用本文复制引用
何彩,王煊,邓明明,周正端,刘宏,屈小平..银杏叶提取物对大鼠重症急性胰腺炎肾损伤的保护作用及机制研究[J].重庆医学,2011,40(29):2952-2954,2958,封3,封4,6.
