广西植物2011，Vol.31Issue(4)：520-525,549,7.DOI:10.3969/j.issn.1000-3142.2011.04.019
薇甘菊可溶性蛋白和抗氧化酶活性对田野菟丝子不同寄生密度的响应
Responses of Mikania micrantha to parasitization of Cuscuta campestris in total soluble protein content and activities of antioxidant enzymes
摘要
Abstract
To develop efficient biocontrol techniques for the invasive weed Mikania micrantha,the use of the obligate parasitic plant,Cuscuta campestris Yuncker as a biological control was investigated.In this experiment,whether the impacts of the parasite on host soluble protein content and activities of some antioxidant enzymes were affected by the density of the parasite was tested.The responses of M.micrantha to parasitic densities of 0,1,2,4 and 8 individual seedlings of C.campestris per host plant were examined.On the 30th day of parasitization,infection with more than 1C.campestris seedling significantly lowered soluble protein content.Compared with control,activities of superoxide dismutase (SOD) and peroxidase (POD) significantly increased at parasitic density of 1 parasite per host plant;but as parasitic density increased,both SOD and POD activities decreased.Moreover,SOD activity was not changed but POD activity was significantly decreased at parasitic density of 4 parasites per host,and both SOD and POD activities were significantly decreased at parasitic density of 8 parasites per host,compared with control.The infected plants had significantly lower catalase (CAT) activity but higher SOD/CAT,SOD/POD and SOD/ (CAT +POD) ratios than the control at all parasitic densities.The results indicated that the effects of C.cam pestris infection on M.micrantha were density dependent,which provided a basis for refining this strategy for biological control of M.micrantha.The optimal cost-effective number of parasites to control M.micrantha was 4 per host plant in the field.关键词
生物防治/入侵杂草/寄生植物/抗氧化酶Key words
biological control/ invasive weed/ parasitic plant/ antioxidant enzymes分类
农业科技引用本文复制引用
刘梦佼,洪岚,沈浩,韦霄,叶万辉,曹洪麟..薇甘菊可溶性蛋白和抗氧化酶活性对田野菟丝子不同寄生密度的响应[J].广西植物,2011,31(4):520-525,549,7.基金项目
Foundation items:Supported by National Basic Research Program of China“973”Program(2009CB119200) （2009CB119200）
the Knowledge Innovation Program of the Chinese Academy of Sciences （）
the National Natural Science Foundation of China(30470345) （30470345）
Guangdong Natural Science Foundation(05200701) （05200701）
