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一测多评法测定博落回果实中原阿片碱、别隐品碱、血根碱和白屈菜红碱的含量

李文亮 曾建国 刘发宝 张雪

湖南中医药大学学报2011,Vol.31Issue(7):25-28,4.
湖南中医药大学学报2011,Vol.31Issue(7):25-28,4.DOI:10.3969/j.issn.1674-070X.2011.07.007.025.04

一测多评法测定博落回果实中原阿片碱、别隐品碱、血根碱和白屈菜红碱的含量

Contents determination of protopine, allocryptopine, sanguinarine and chelerythrine in fruit of Macleaya cordata with method of multi-assay depending on one determination

李文亮 1曾建国 2刘发宝 1张雪3

作者信息

  • 1. 湖南中医药大学,湖南长沙410208
  • 2. 湖南省中药提取工程研究中心,湖南长沙410331
  • 3. 湖南农业大学,湖南长沙410007
  • 折叠

摘要

Abstract

Objective To set up a method of multi-assay depending on one determination to detect the contents of umarine, allocryptopine, sanguinarine and chelerythrine in fruit of Macleaya cordata (Willd.)R.Br. Methods The fruit of Macleaya cordata was studied to set up the relative correction factors of allocryptopine, sanguinarine and chelerythrine for detecting protopine, and the factors were used to measure the contents of allocryptopine, sanguinarine and chelerythrine, and then, the method was set up. At the same time, other measurements used to detect the contents of the four alkaloids, comparing the two methods to validate the accuracy and feasibility of the new method. Results Four alkaloids contents in 9 batches of Macleaya cordata were used the new method to determine, and there were no significant differences. Conclusion The new method is feasible and accurate to evaluate the contents of the four alkaloids in fructus of Macleaya cordata.

关键词

博落回/生物碱/一测多评/相对校正因子/高效液相色谱法

Key words

Macleaya cordata R.Br. (WilId.)/ alkaloids/ multi -assay depending on one determination/related correction factor(RCF)/HPLC

分类

医药卫生

引用本文复制引用

李文亮,曾建国,刘发宝,张雪..一测多评法测定博落回果实中原阿片碱、别隐品碱、血根碱和白屈菜红碱的含量[J].湖南中医药大学学报,2011,31(7):25-28,4.

基金项目

国家科技部国际合作与交流专项(2009DFA31270) (2009DFA31270)

湖南中医药大学学报

OACSTPCD

1674-070X

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