湖南中医药大学学报2011，Vol.31Issue(7)：74-76,3.DOI:10.3969/j.issn.1674-070X.2011.07.023.074.03
大鼠骨髓间充质干细胞分离和培养方法的研究
Study on isolation and culture of rat bone marrow mesenchymal stem cells
摘要
Abstract
Objective To explore a purification and culture method of bone marrow mesenchymal stem cells (BMSCs) in rat in vitro. Methods BMSCs from bone marrow of 3～4 week old rats were separated and purified by a improved method of whole bone marrow adherent. The morphological changes of BMSCs were observed continuously under the microscope. The expression of surface antigens CD45 and CD29 were analyzed by flow cytometry. Results Cultured primary BMSCs were circular or spindle -shaped and the most BMSCs were adherent within 24 hours, and 80% ～90% BMSCs were in fusion within 8 -10 days. The Transfer of generation cycle was 6～8 days after purification. The Flow cytometry identification showed that CD29 was positive and CD45 negative. Conclusion The improved method of whole bone marrow adherent, can successfully isolate and culture BMSCs in rats, which is proved to be an optimal methods for cell preparation and culture.关键词
骨髓间充质干细胞/细胞培养/大鼠Key words
bone marrow mesenchymal stem cells(BMSCs)/cell culture/rats分类
生物科学引用本文复制引用
谭琦,黄政德,王立凤..大鼠骨髓间充质干细胞分离和培养方法的研究[J].湖南中医药大学学报,2011,31(7):74-76,3.基金项目
国家自然科学基金项目(30973750) （30973750）
湖南省教育厅重点项目(09A071) （09A071）
2010年湖南省研究生科研创新项目(CX2010B341). （CX2010B341）
