激光生物学报2011，Vol.20Issue(4)：490-495,6.DOI:10.3969/j.issn.1007-7146.2011.04.012
沉默FNBP1基因对人HL-7702细胞 体外增殖及迁移能力的影响
Effects of Down-regulating FNBP1Gene on Cell Proliferation and Metastasis Ability in HL-7702 Cells
摘要
Abstract
To investigate the effect of silencing FNBPX (formin-binding protein 1) gene on the proliferation and metastasis ability of HL-7702 cells, Three small interference expression vectors (Si-1 ,Si-2,Si-3) specific to FNBPX mRNA were designed and constructed, then were transfected into the HL-7702 cells after identified by restriction analysis and DNA sequencing. Analysis of efficiency of FNBPX siRNA was detected by RT-PCR and Westem-blot to choose the highest inhibitory target of FNBP1 among the three vectors. The cell line with stable expression of siRNA of FNBPX was selected by G418 medium for 15 days. After FNBPX knockdown, the proliferation of HL-7702 cells was measured by the XTT assay and metastasis ability was measured by wound healing assay. Results are as following, restriction analysis and sequencing proved that siRNA expression vector was constructed correctly; FNBPX mRNA and protein expression obviously decreased in HL-7702 cells transfected with siRNA ( Si-1、 Si-2 、 Si-3 ) respectively compared with the control groups, and Si-2 have the highest inhibitory effect. XTT showed that Si-2 can significantly inhibit the proliferation in HL-7702 cells, and have no effect on metastasis ability of HL-7702, which strongly indicated that FNBP1 plays an important role in proliferation of HL-7702 cells.关键词
FNBP1/RNA干扰/HL-7702细胞Key words
FNBP1/ RNA interference/ HL-7702 cell分类
生物科学引用本文复制引用
张乾英,王蕴红,朱重阳,张军..沉默FNBP1基因对人HL-7702细胞 体外增殖及迁移能力的影响[J].激光生物学报,2011,20(4):490-495,6.基金项目
国家自然科学基金项目(20803098) （20803098）
重庆市教育委员会科学技术研究项目(KJ080301) （KJ080301）
