解剖学杂志2011，Vol.34Issue(4)：514-517,4.
督脉电针联合神经干细胞移植治疗脊髓全横断损伤后aggrecan的表达变化
Effects of treatment of combination of goremor vessel electroacupuncture and transplanted neural stem cells on expression of aggrecan after spinal cord injury
赵伟1
作者信息
- 1. 滨州医学院组织学与胚胎学教研室,烟台264003
- 折叠
摘要
Abstract
Objective: o explore the effects of combination of goremor vessel electroacupuncture and transplanted neural stem cells (NSCs) on the expression of aggrecan of chondroitin sulfate proteoglycans in the injured tissues of rat spinal cord transected completely. Methods: Adult rats were divided into control group, goremor vessel electroacupuncture group (EC group), neural setm cells transplanted group (NSCs group) and goremor vessel electroacupuncture plus NSCs transplanted group (EC+NSCs group). All animals were performed complete transection between T9 and T10 segment of the spinal cord. The electroacupunctural treatment started at the postoperative day among EC group and EC+NSCs group. Aggrecan immunohistochemical staining was made in the tissues of the injured spinal cord. The immuno-positive gray scale of immuno-reactivity was detected with computer image analysis technique. Results: The expression of aggrecan at cranial segment and caudal segment of injured spinal cord segment was increased more slightly in the control group than in the normal group. Fourteen days after operation, the expression of aggrecan at the cranial segment and caudal segment of the injured spinal cord segment was decreased more slightly in EC group, NSCs group and EC+NSCs group than in the control group. Twenty-eight days after operation, the expression of aggrecan at the injured segment maintainted a lower level in EC group, NSCs group and EC+NSCs group; The expression of aggrecan was more prominent in EC group and EC+NSCs group. Conclusion: The combination of goremor vessel electroacupuncture and transplanted neural stem cells can significantly reduce the expression of aggrecan, which is an inhibitor of axon regeneration after spinal cord injury (SCI). This effect may be one of the mechanism that the combination therapy of both can promote the functional recovery after SCI.关键词
督脉电针/神经干细胞移植/脊髓损伤/aggrecan/硫酸软骨素蛋白多糖/大鼠Key words
goremor vessel electroacupuncture/ neural stem cells transplantation/ spinal cord injury/ aggrecan/ chondroitin sulfate proteoglycans/ rat引用本文复制引用
赵伟..督脉电针联合神经干细胞移植治疗脊髓全横断损伤后aggrecan的表达变化[J].解剖学杂志,2011,34(4):514-517,4.
