解剖学杂志2011，Vol.34Issue(4)：462-464,475,4.
槲皮素诱导人宫颈癌Hela细胞凋亡的作用
Quercetin induces apoptosis of human cervical carcinoma Hela cells
摘要
Abstract
Objective: To investigate the cell growth inhibition and apoptosis induced by quercetin on human cervical carcinoma Hela cells and the role of mitochondrium membrane potential in the process of apoptosis. Methods: The cell growth inhibiting ratio of Hela cells was assessed by the acid phosphatase assay. Morphologic changes of the quercetin-treated Hela cells were observed by fluorescence microscopy after acridine orange/ethidium bromide (AO/EB) staining. The cell mito-chondrial membrance potential was detected by flow cytometry. Results: Quercetin inhibited the proliferation of Hela cells significantly, and the growth inhibitory rate presented time and dose-dependent relationship. Typical morphological changes of cell apoptosis were observed after the Hela cells had been exposed to quercetin for 48 hours by the using AO/EB staining method. The cell mitochondrial trans-membrance potentials in Hela cells was declined after being treated with 100 μmol/L quercetin for 24 and 48 hours compared with the control group (P<0. 01). Conclusion: Quercetin can inhibit the proliferation of Hela cells,and induce cell apoptosis by declining trans-membrance potentials of mitochondrium.关键词
槲皮素/宫颈癌细胞/细胞凋亡Key words
quercetin/ Hela cells/ cell apoptosis引用本文复制引用
马朋,宋晓冬,闫苗苗,牛新华,于敏..槲皮素诱导人宫颈癌Hela细胞凋亡的作用[J].解剖学杂志,2011,34(4):462-464,475,4.基金项目
山东省自然科学基金(ZR2009EM006) （ZR2009EM006）
