解剖学杂志2011，Vol.34Issue(4)：493-495,3.
伊文思蓝检测急性高眼压后大鼠血-视网膜屏障的变化
Changes of blood-retinal barrier of rat following acute high intraocular pressure using Evans blue*
摘要
Abstract
Objective:To detect the changes of blood-retinal barrier (BRB) following acute high intraocular pressure (AHIOP) in a rat model. Methods: The rats were randomly divided into normal groups, experimental control groups and experimental groups. The rats of the experimental groups were made into AHIOP model, and then survived for 3 h, 12 h, 1 d, 3 d and 7 d. 3% Evans blue (EB) was injected into the saphenous vein of rats before they were sacrificed. The distribution of EB in the retinal wholemount and retinal slice was observed by using confocal microscopy and the EB content of retina was measured by using spectrophotometer. Results: EB red fluorescence spots were mainly distributed in the ganglion cell layer around capillaries following AHIOP. In 3 h, 12 h and 1 d, it was also found in the inner nuclear layer surrounding the capillaries. The EB red fluorescence spots of the central retina began to increase in 3 h following AHIOP, reached its peak in 12 h, and then gradually decreased. Most of them in the peripheral retina were found in 3 h after AHIOP, and then gradually decreased. Further quantitative analysis showed the same spatial and temporal changes. Conclusion : BRB damage of rats is more evident in its early stage after AHIOP, and restores gradually in its late stage. The serious breakdown of BRB in the peripheral retina precedes the central retina. BRB damage is mainly distributed in the ganglion cell layer.关键词
急性高眼压/伊文思蓝/血-视网膜屏障/大鼠Key words
acute high intraocular pressures Evans blue/blood-retinal barrier/ rat引用本文复制引用
张全鹏,王慧,童建斌,陈旦,周利红,黄菊芳..伊文思蓝检测急性高眼压后大鼠血-视网膜屏障的变化[J].解剖学杂志,2011,34(4):493-495,3.基金项目
青年国家自然科学基金(30800591) （30800591）
