山东医药2011，Vol.51Issue(36)：25-26,2.
体外人脐静脉内皮细胞的培养鉴定及抗原表达分析
Experimental study on culture, identification, antigens analysis of human umbilical vein endothelial cells in vitro
摘要
Abstract
Objective To study the isolation, culture and identification of human umbilical vein endothelial cells (HUVECs) in vitro, and analyze antigens expression. Methods Cultured cells were isolated from umbilical cord by enzyme digestion and were cultured in vitro. Cultured cells were subjected to flow cytometer to analyze the expression of CD31, CD34, VWF, KDR and CD133. Immunofluorescence was performed to detect VWF and dual binding of ac-LDL and UEA-1. Results Cultured HUVECs were like slabstones, which were positively stained for CD31, CD34, VWF, KDR, but negative for CD133. Cultured HUVECs could endocytose ac-LDL and UEA-1. Conclusions HUVECs with the characters of endothelial cells can be cultured abundantly by this method. Purity of HUVECs can be assayed by analyzing antigens expression, which can be used to establish the model of endothelial cells.关键词
脐静脉内皮细胞/流式细胞术/抗原表达Key words
human umbilical vein endothelial cells/flow cytometry/antigen express分类
医药卫生引用本文复制引用
刘长乐,郑心田,李广平,孟恒星,邱录贵..体外人脐静脉内皮细胞的培养鉴定及抗原表达分析[J].山东医药,2011,51(36):25-26,2.基金项目
天津市科技发展计划基金资助项目(0438031111) （0438031111）
