山东医药2011，Vol.51Issue(36)：27-29,3.
红系造血分化中分化调节因子的表达及调控
Experession and regulation of erythroid differentiation factor in erythrod differentiation
摘要
Abstract
Objective To investigate the expression of erythroid differentiation regulate factor (EDRF) in normal he-matopoitic tissue and erythroleukemia cells, and to explore the mechanism of EDRF regulation. Methods The expression of EDRF in erytholeukemia cell NF-E2, HB22.2 and in mice fetus liver tissue was detected by Northern blot method, the DNA transmutation was detected by Southern blot method. The gradual change of EDRF expression during erythoid differentiation was observed by using erythroleukemia cell HB60-5 which induced differentiation by changing culture. To observe the effects of erythroid factors GATA-1, NF-E2 and Fli-1 on EDRF expression by using transgenic technique. Results The transfection of NF-E2 into CB3 and HB22.2 could restore the expression of EDRF. During the erythroid differentiation of HB60-5 cell, the NF-2, GATA-1, α-globin and EDRF expression were gradually evaluated simultaneously. In HB60-5 cell, transfection of GATA-1 could promote the expression of EDRF, in contrast, the transfection of Fli-1 suppressed the expression of EDRF. Conclusions EDRF was erythroid specific gene which was high expressed in normal erythroid tissue , however in erythroleukemia cells the high expression of EDRF was greatly suppressed which was not caused by DNA aberration. EDRF could be up-regulated by erythroid factor GATA-1 and NF-E2, and down-regulated by Fli-1.关键词
红系分化调节因子/红系造血分化/基因调控Key words
erythroid differentiation regulate factor/erythroid differentiation/gene regulation分类
医药卫生引用本文复制引用
谭业辉,王畅,王冠军..红系造血分化中分化调节因子的表达及调控[J].山东医药,2011,51(36):27-29,3.基金项目
吉林省科技厅资助项目(20080135) （20080135）
