天然产物研究与开发2011，Vol.23Issue(4)：638-642,5.
大黄素对顺铂耐药卵巢癌细胞的逆转作用及其相关基因表达研究
The Reversal Effect of Emodin on Cisplatin Resistance in Ovarian Cancer Cells and the Expression of Resistance-associated Genes
摘要
Abstract
To investigate the drug resistance reversal effect and mechanism of emodin in cisplatinum-resistant ovarian cancer cells SKOV3/DDP (short for cisplatin) in vitro and study the expression of HIF-lα,STATl ,and GSTP1 resistance-associated genes. Methyl thiazolyl tetrazolium ( MTT) method was used to detect the reversal index ( RI) of SK-0V3/DDP cells and the reversal effect of emodin. The resistance-associated genes expression of HIF-lα,CK2α,STATl, and GSTP1 was detected by Real time PCR. The results showed that noncytotoxic dose of emodin reversed the cisplatin-resistance of SKOV-3/DDP,the reversal index (RI) was 1.91 and 1.30 respectively for 7.8 mg/L and 3.9 mg/L emodin. Compared with SKOV3 cells,expression level of HIF-l<x,STATl ,CK2α,and GSTP1 mRNA in SKOV3/DDP was significantly raised(P <0.01). 3.9 mg/L and 7. 8 mg/L emodin could down regulated the gene expression of HIF-lα, CK2α,and STAT1 in a dose-dependent manner. Though 7. 8 mg/L emodin down-regulated GSTP1 mRNA expression, 3.9 mg/L emodin had no such effects. The mRNA expression level of those gene was significantly down-regulated when 7. 8 mg/L and 3.9 mg/L emodin was combined with DDP (P <0.01). Emodin can down regulate the gene expression of HIF-la,CK2a,STATl ,and GSTP1 on SKOV-3/DDP cells,which may account for the reversal effect of emodin on cisplatin resistance in ovarian cancer cells.关键词
大黄素/卵巢癌耐顺铂细胞/耐药逆转/HIF-1α/STAT1/GSTP1/CK2αKey words
emodin/cisplan'n-resistant ovarian cancer cells SKOV-3/DDP cell line/resistance reversal/HIF-1 α/STAT1/ GSTPl/CK2α分类
医药卫生引用本文复制引用
秦春明,侯华新,程道海,李力,黎丹戎,刘瑛,宋慧..大黄素对顺铂耐药卵巢癌细胞的逆转作用及其相关基因表达研究[J].天然产物研究与开发,2011,23(4):638-642,5.基金项目
国家自然科学基金资助项目(30660047) （30660047）
广西自然科学基金资助项目(0728112) （0728112）
广西研究生教育创新计划资助项目(2007105981007M09) （2007105981007M09）
