生物加工过程2011，Vol.9Issue(5)：11-16,6.DOI:10.3969/j.issn.1672-3678.2011.05.03
生物法合成辛伐他汀
Biosynthesis of simvastatin
摘要
Abstract
Simvastatin was synthesized from Monacolin J using acyltransferase LovD. The objective of this study was to investigate the expression of acyltransferase LovD, and optimize its medium and fermenting conditions. The level of expression was visualized by sodium dodecyl sulfate-poryacryl-amide gel electro-phoresis. To quantify the active amount of LovD expressed under different growth conditions and in different media, an activity assay was used. The optimum fermentation conditions of acyltransferase LovD were determined as follows:fermentation medium was Terrific Broth; inoculation volume was 4% ; ceD density ( 0.0,500 ) before induction was 0. 7; concentration of IPTG was 0. 2 mmol/L; inoculation tempreture was 20 t, and total time of inoculation was 20 h. Under the conditions, the expression level of acyltransferase LovD was 100 mg/L and the yield of simvastatin was 1. 2 g/L.关键词
酰基转移酶/Monacolin J/辛伐他汀Key words
acyltransferase/ Monacolin J/ simvastatin分类
生物科学引用本文复制引用
朱丽平,任宇红,孙常磊,周剑平,魏东芝..生物法合成辛伐他汀[J].生物加工过程,2011,9(5):11-16,6.基金项目
国家高技术研究发展计划(863计划)资助项目(2008AA02Z204) （863计划）
