中国当代儿科杂志2011，Vol.13Issue(9)：751-754,4.
黄芪甲甙对急性病毒性心肌炎小鼠IGF-1及相关蛋白表达的作用研究
Effects of astragaloside on IGF-1 and associated protein expression in mice with acute viral myocarditis
何春枝 1李双杰2
作者信息
- 1. 武汉市普爱医院儿科,湖北,武汉,430034
- 2. 湖南省儿童医院感染科,湖南,长沙,410007
- 折叠
摘要
Abstract
Objective To study the effects of astragaloside on the expression of insulin-like growth factor-1 (ICF-1 ) and associated proteins in mice with viral myocarditis. Methods Sixty-five 4-week-old BALB/C mice were randomly divided into 5 groups: normal control, astragaloside control, untreated myocarditis, low-dose and high-dose astragaloside-treated myocarditis. The BALB/C mice in the later three groups were intraperitoneally injected with CVB3. The low-dose and high-dose astragaloside-treated myocarditis groups were given astragaloside of 0.07 and 0.6 mg/kg ? D, respectively by intragastric administration. Fifteen days later, the samples of blood and muscular tissues were obtained. The expression of ICF-1 in plasma was measured using ELISA. The levels of ICF-1 and associated proteins in muscular tissues were measured by immunohistochemistry. The expression of IGF-1 mRNA in muscular tissues was examined by RT-polymerase chain reaction ( RT-PCR). Results The expression of IGF-1 and associated proteins increased significantly in mice infected with CVB3. High-dose astragaloside treatment reduced the expression of ICF-1 and associated proteins, but low-dose astragaloside did not. Conclusions High-dose astragaloside may reduce the expression of ICF-1 and associated proteins in mice with acute viral myocarditis, possibly thus providing protective effects on muscular tissues.关键词
病毒性心肌炎/黄芪甲甙/胰岛素样生长因子-1/小鼠Key words
Viral myocarditis/ Astragaloside/ Insulin-like growth factor-1/ Mice分类
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何春枝,李双杰..黄芪甲甙对急性病毒性心肌炎小鼠IGF-1及相关蛋白表达的作用研究[J].中国当代儿科杂志,2011,13(9):751-754,4.
