中国美容医学2011，Vol.20Issue(9)：78-80,3.
环孢菌素对大鼠牙龈结缔组织胶原及TIMP-1蛋白表达影响的实验研究
Experimental research of the effect of Cycloporine on the expression of collagen and TIMP-1 in connective tissue of rats
摘要
Abstract
Objective To observe the effect of Cyclosporine (CsA) on collagen and expression of the collagenase TIMP-1 in gingival connective tissue of rats. To approach the collagen metabolic mechanism of CsA-induced gingival connective tissue overgrowth. Methods Eighty SPF grade male 7 weeks old Wistar rats were randomly divided into experimental group and control group medially, and each group was divided into 4 subgroups according to the duration of treatment (10,20,30 and 40 days). The experimental objects were given intragastric administration of fresh milk including CsA.and the control ones were given intragastric administration of fresh milk the same as the experimental objects. After giving perfusion 4% paraform trans -heart to internal fixation, the specimens were get and made buccolingual paraffin sections at lower first molar. Collagen was detected using Masson trichrism improvement, and the expression of TIMP-1 using immunohistochemisty of PV. Results Collagenic number of the experimental group were significantly increased.and the collagenic diameter were significantly thickening. The expression of TIMP-1 of the experimental group were significantly up-regulated. Conclusion CsA-induced gingival connective tissue overgrowth is likely to associated with the up-regulated expression of the collagenase TIMP-1 and accumulating of collagen.关键词
环孢菌素/药物性牙龈增生/结缔组织/胶原/TIMP-1Key words
Cyclosporine/drug-induced gingival overgrowth/ connective tissue/ collagen/ TIMP-1分类
生物科学引用本文复制引用
马肃,刘培红,李延武,刘薇婷,于洋,王喜华,李莹..环孢菌素对大鼠牙龈结缔组织胶原及TIMP-1蛋白表达影响的实验研究[J].中国美容医学,2011,20(9):78-80,3.基金项目
黑龙江省教育厅科学技术研究项目(编号:11521111) （编号:11521111）
